Abstract
In this investigation, a new planar chromatography method was developed for fingerprinting the extracts of Deoni cattle urine and Acalypha indica leaf samples. The researchers used a polar solvent gradient that included various combinations of ethanol, methanol, and chloroform. Optimal phytochemical separation was achieved on thin-layer chromatography silica gel plates. Freshly collected urine samples were distilled and concentrated, whereas shade-dried plant samples were subjected to Soxhlet extraction. Under UV illumination (254 nm), unique Rf values were obtained, thus indicating a significant amount of phenolic compounds, flavonoids, tannins, and alkaloids.
